The Department of Chemistry and Biochemistry Stockroom consists of a complex of five rooms in the northwest corner of the ground floor of Ebaugh Laboratories. In addition to storage, the stockroom also functions to prepare solutions and reagents for the chemistry laboratories. If you are in need of a chemical, there is a online search engine available to Denison campus only which will help you determine if the stockroom has what you need. For the chemical inventory search engine, desk lists, and links to safety materials, please visit the Chemistry & Biochemistry Channel on MyDenison.
A number of instruments are available for laboratory instruction and research. Use of instrumentation is integral to the student experience at all levels. Information is given here about the specific instrumentation our students and faculty have experience with and is not meant an endorsement of a particular vendor. Most of the instruments are interfaced to computers which have network access.
Detailed list of equipment
- Five Hewlett-Packard HP-8452A Specrtrophotometers equipped with photodiode array detection are installed on carts and are used in laboratories at all levels of the curriculum (and of the building).
- A new Uvicon 9500 Dual Beam UV-Vis Spectrophotometer is also available for specialized applications.
- Ten UNICO 2800 UV/Vis Spectrophotometers and a BIO-TEK EL 800 Universal Microplate Reader are used primarily in the Biochemistry Labs.
- Two Molecular Devices Spectromax 250 Scanning Microplate Spectrophotometers are available for high-through put UV-VIS applications.
- The department is equipped with an Ocean Optics USB-2000-FL-450 Fluorescence Spectrometer with a PX-2 Pulsed Xenon light source that is used primarily in environmental chemistry and research.
- A dual-monochromator TECAN Infinite M200 Pro Microplate Spectrophotometer for high-throughput absorbance, luminescence and fluorescence applications.
Gas Chromatography/Mass Spectrometry
- Three Agilent 6850 GC's with model 3395 integrators are installed on carts for use in research and throughout the curriculum. This system is among the GC systems described on the
- Agilent Website
- An Agilent 6850 GC is equipped with a 5973 MSD. This system is used at all levels of the curriculum and in research.
- A Varian Model 3380 gas chromatograph equipped with thermal conductivity and flame ionization detectors is used heavily in the analytical chemistry course. This system is among the GC systems described on the Varian Website.
Nuclear Magnetic Resonance Spectroscopy
- The department operates a 400 MHz Bruker Avance Nuclear Magnetic Resonance (NMR) spectrometer. The NMR is equipped with a robotic autosampler, which allows us to operate the instrument unattended.Students process spectra remotely at one of the department's microcomputers. This system was installed in May 2003; a description of it can be found on the
- Bruker Website
- The department operates a Bruker Vector-22 FT-IR which is equipped with a variety of cells to provide flexibility in the types of samples that can be handled. Used primarily in organic chemistry teaching labs and in research, our FT-IR is installed on a cart to accommodate applications throughout Ebaugh Labs.
High Performance Liquid Chromatography
- A Waters HPLC system that includes a Multisolvent Delivery System with UV detection (model 2487) and fluorescence detection (Shimadzu, RF-535), two model 515 pumps and a model 600E pump and controller, is used primarily in teaching and research in biochemistry.
Atomic Absorption Spectrometry
- The department operates a Varian SpectrAA 100 Atomic Absorption Spectrometer, manufactured by Varian Associates Incorporation. It is used in the Analytical Chemistry laboratory and supports projects in the first year course as well as research projects.
- Although it can be used for a variety of chemical and biochemical experiments, the main use of the thermal cycler is for PCR -- the Polymerase Chain Reaction. The department has an Eppendorf Mastercycler Thermal Cycler and a Techne Peltier-effect thermal cycler.
- The department is equipped with a number of centrifuges for a range of speeds, temperatures and sample sizes, including a Beckman J2-HS High-Speed Centrifuge, an Eppendorf 5804R Multipurpose Centrifuge and an Eppendorf 5417R Benchtop Centrifuge.
- A Thermo Electron French Press Cell Disrupter and a Fisher Scientific 550 Sonic Dismembrator are used in cell preparation for research and advanced classes.
Synthesis of Air Sensitive Compounds
- The department has a Vacuum Atmospheres Company dry box. Dr. Jordan Fantini’s research group is using the dry box for inorganic chemistry research of the calixarene. The dry box provides an inert atmosphere that is best suited for the hygroscopic chemicals used in the research.
- The department operates a Burleigh Instrumentation Scanning Tunneling Microscope (STM) and three Atomic Force Microscopes (AFM),Topometrix TMX 2010, TMX 2000 Discoverer, and TMX 1010 Explorer.
- The Chemistry and Biochemistry Department and the Biology Department jointly operate an Alpha Innotech FluorChem HD2 advanced imaging system.This system has a cooled CCD camera and is primarily used for imaging chemiluminescence and fluorescence of gels and blots in biochemical research.
- Besides several computers interfaced to specific instruments, many computers are available for other applications.
- The department operates two Silicon Graphic's Unix Workstations, with 5 PC clients, for molecular modeling. For analysis of small molecular structures, the program Spartan is used, while the programs Quanta/CHARMm are used to analyze biochemical structures. With large monitors and lots of computer memory, this network is ideal for modeling structures both large and small.
- For data acquisition in the general chemistry, analytical chemistry and physical chemistry laboratories, the department has 24 new Vernier LabQuest Handheld Data Acquisition systems.
- All three instructional spaces in Ebaugh Labs are equipped with document cameras, DVD capabilities and video-projection from networked laptops or desktop PC's.
- A set of 14 Dell Notebooks on a wireless network can be used anywhere in Ebaugh Labs. These laptops support both classroom and laboratory instruction as well as research. The entire Denison campus is provided with wireless internet coverage.
- A computer cluster located in the departmental library provides students access to specialized scientific software and the internet.